• Tetiana Kravets Production Manager at “Simesta Production Firm”
  • Oleksii Harieiev director of “Simesta Production Firm”
  • Kateryna Lobanova head of the serological department at “Simesta Production Firm”
Keywords: environmental peptides, phlogogen of allergic inflammation, catalytic activity of proteolytic enzymes, protein hydrolysis


The purpose of this work was to experimentally confirm the concept of the inflammatory nature of allergic reactions, where the phlogogen is environmental peptides with preserved
epitope sequences due to incomplete hydrolysis to amino acids. The resulting effect is associated with congenital or acquired selective fermentopathy of external proteolytic enzymes
of the microbiome.

Materials and methods. The experiment involved 22 strains of microorganisms that make up the transient and normoflora of the human intestine, forming a group of probiotics, widely used as biologically active additives (BAA) in various formulations as a means of therapy. The catalytic action of external proteolytic enzymes of the studied microorganisms in the hydrolysis reactions of macronutrient proteins in vitro was evaluated. The cultivation of microorganisms was carried out on selective nutrient media with added extracts of cow’s milk proteins, chicken eggs, soybeans, wheat, and peanuts. The hydrolysis products were fixed using polyacrylamide gel electrophoresis. As a control, the result of electrophoresis of the components of the extracts of the studied proteins was taken into account.

Results and its discussion. In a fairly large number of cases, the catalytic activity of enzymes of microorganisms from various taxonomic groups in the reaction of hydrolysis of one specific protein is noted, which gives reason to regard this phenomenon as a diversity factor that ensures the stability of such an ecosystem as the human microbiome. It is also important to take into account that the enzymatic activity of closely related microorganisms in the hydrolysis of proteins is not of the same type, which makes it possible to obtain
hydrolysis products as a source of microbial metabolism from a plurality of various proteins. This phenomenon may be of the adaptive nature of food synergy.

Conclusions. There are reasons to regard the manifestations of allergy as one of the variants of the inflammatory reaction, i.e. syndrome, a set of symptoms of inflammation accompanying a
somatic or functional disease, in the pathogenesis of which there is enzymopathy for individual proteolytic enzymes.


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How to Cite
Kravets, T., Harieiev, O., & Lobanova, K. (2023). PROTEOLYSIS IN THE PATHOGENESIS OF ALLERGIC DISEASE. Men’s Health, Gender and Psychosomatic Medicine, (1-2(14-15), 23-39.