PROTEOLYSIS IN THE PATHOGENESIS OF ALLERGIC DISEASE
The purpose of this work was to experimentally confirm the concept of the inflammatory nature of allergic reactions, where the phlogogen is environmental peptides with preserved
epitope sequences due to incomplete hydrolysis to amino acids. The resulting effect is associated with congenital or acquired selective fermentopathy of external proteolytic enzymes
of the microbiome.
Materials and methods. The experiment involved 22 strains of microorganisms that make up the transient and normoflora of the human intestine, forming a group of probiotics, widely used as biologically active additives (BAA) in various formulations as a means of therapy. The catalytic action of external proteolytic enzymes of the studied microorganisms in the hydrolysis reactions of macronutrient proteins in vitro was evaluated. The cultivation of microorganisms was carried out on selective nutrient media with added extracts of cow’s milk proteins, chicken eggs, soybeans, wheat, and peanuts. The hydrolysis products were fixed using polyacrylamide gel electrophoresis. As a control, the result of electrophoresis of the components of the extracts of the studied proteins was taken into account.
Results and its discussion. In a fairly large number of cases, the catalytic activity of enzymes of microorganisms from various taxonomic groups in the reaction of hydrolysis of one specific protein is noted, which gives reason to regard this phenomenon as a diversity factor that ensures the stability of such an ecosystem as the human microbiome. It is also important to take into account that the enzymatic activity of closely related microorganisms in the hydrolysis of proteins is not of the same type, which makes it possible to obtain
hydrolysis products as a source of microbial metabolism from a plurality of various proteins. This phenomenon may be of the adaptive nature of food synergy.
Conclusions. There are reasons to regard the manifestations of allergy as one of the variants of the inflammatory reaction, i.e. syndrome, a set of symptoms of inflammation accompanying a
somatic or functional disease, in the pathogenesis of which there is enzymopathy for individual proteolytic enzymes.
Antypova T., Felik S., Kuznetsov V. A new generation of hypoallergenic baby food // Dairy industry.2013. № 12. S. 56–57.;
Holovach T., Kurchenko V. Allergenicity of milk proteins and ways to reduce it // Proceedings of Belarus. state. un. Ser.: Physiological, biochemical and molecular bases of functioning of biosystems.2010. T. 5. Ch. 1. S. 9–51.;
Kruchinin A. Development of technology for joint coagulation of milk and vegetable proteins in the production of cheese product with reduced allergenicity: Moscow, 2014.;
Bogdanova E. Development of physicochemical and biotechnological methods of modification of milk whey protein-carbohydrate complex for implementation in food production. “Voronezh State University of Engineering Technologies” https://www.ncfu.ru/export/uploads/dokumenty-dissertacii/disser_bogdanova.pdf
Golovach TN, Kurchenko VP Hydrolysis of milk proteins by enzyme preparations and systems of lactic acid bacteria “Institute of Meat and Dairy Industry”, Belarusian State University, Minsk, Republic of Belarus http://www.bio.bsu.by/proceedings/articles/2012-7-1-106-126.pdf
Nitzan Koppel, Vayu Maini Rekdal, Emily P. Balskus. Chemical transformation of xenobiotics by the human gut microbiota Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA. Broad Institute, Cambridge, MA 02139, USA https://www.science.org/doi/10.1126/science.aag2770
Trang T. H. Nguyen, David D. Myrold, Ryan S. Mueller Distributions of Extracellular Peptidases Across Prokaryotic Genomes Reflect Phylogeny and Habitat. Department of Crop and Soil Science, Oregon State University, Corvallis, OR, United States, Department of Microbiology, Oregon State University, Corvallis, OR, United States https://www.frontiersin.org/articles/10.3389/fmicb.2019.00413/full
Lokesh P Tripathi. R. Sowdhamini Genome-wide survey of prokaryotic serine proteases: Analysis of distribution and domain architectures of five serine protease families in prokaryotes https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-9-549
Trang T. H. Nguyen, David D. Myrold,1 and Ryan S. Mueller. Distributions of Extracellular Peptidases Across Prokaryotic Genomes Reflect Phylogeny and Habitat https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411800
Costi D. Sifri Quorum Sensing: Bacteria Talk Sense Division of Infectious Diseases and International Health, University of Virginia Health System, Charlottesville https://academic.oup.com/cid/article/47/8/1070/345306
Marijke Frederix, J. Allan Downie. Quorum Sensing «Достижения микробной физиологии», 2011г. https://www.sciencedirect.com/topics/immunology-and-icrobiology/quorum-sensing
Yarova G.Ya. Bioregulatory functions and pathogenetic role of proteolysis. III. Physiological role and biochemical mechanisms of proteolytic degradation of proteins. - Laboratory medicine №6 RAMLD
Alexander Varshvsky. Regulated protein degradation https://www.cell.com/trends/biochemical-sciences/fulltext/S0968-0004(05)00095-2
Samar A Mahmoud. Peter Chien. Regulated Proteolysis in Bacteria Department of Biochemistry and Molecular Biology, Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, Amherst MA 01003 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013389
Yarovaya G.Ya. The role of proteolytic enzymes in the control of various stages of apoptosis
Jerica Sabotič, Janko Kos. Microbial and fungal protease inhibitors – current and potential applications Applied Microbiology and Biotechnology volume 93, pages1351–1375 (2012 https://link.springer.com/article/10.1007/s00253-011-3834-x
Yu-Zhong Zhang, Wei-Xin Zhang, Xiu-Lan Chen. Mechanisms for Induction of Microbial Extracellular Proteases in Response to Exterior Proteins Authors: AUTHORS INFO & AFFILIATIONS https://journals.asm.org/doi/10.1128/AEM.01036-20
Tomasz Kantyka, Neil D. Rawlings, Jan Potempa. Prokaryote-derived protein inhibitors of peptidases: a sketchy occurrence and mostly unknown function https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2952067/
Mirva Steinhoff, Markus Fischer, Angelika Paschke. Comparison of extraction conditions for milk and hen’s egg allergens. Food Additives and Contaminants, 2011, pp.1. 10.1080/19440049.2010.545957 hal-00670743
Yeung JM, Newsome WH, Abbott MA. 2000. Determination of egg proteins in food products by enzyme immunoassay. JAOAC Int. 83:139-143.
S. J. Koppelman, M. Smits, M. Tomassen, et al. Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions – Ara h2 and Ara h6 Are Readily Bio-Accessible Nutrients. 2018 Sep; 10(9): 1281;
J. R. Soares, I. M.Guimarães, et al. Allergen Extraction: Factors Influencing the Yield, Allergenicity and Sensitivity of Immunoassays)
Laemmli (Laemmli UK. Cleavage of structural proteins during the a assembly of the 439 head of bacteriophage T4. nature. 1970;227(5259):680-5.)
Zehra Esra Ilhan,a,b,c Andrew K. Marcus,a Dae-Wook Kang, Bruce E. Rittmann, Rosa Krajmalnik. Browncorresponding pH-Mediated Microbial and Metabolic Interactions in Fecal Enrichment Cultures Garret Suen, EditorGarret Suen, University of Wisconsin – Madison; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5415631
Rubén Sánchez-Clemente, María Isabel Igeño, Ana G. Población, María Isabel Guijo, Faustino Merchán, Rafael Blasco Study of pH Changes in Media during Bacterial Growth of Several Mediumal Strains Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Veterinaria, and Instituto Universitario de Investigación de Carne y Productos Cárnicos (IProCar), Universidad de Extremadura, 10003 Cáceres, Spain; https://www.mdpi.com/2504-3900/2/20/1297/pdf
E.A. Smith, G.T. Macfarlane. Enumeration of amino acid fermenting bacteria in the human large intestine: effects of pH and starch on peptide metabolism and dissimilation of amino acids FEMS Microbiology Ecology, Volume 25, Issue 4, April 1998, Pages 355–368 https://academic.oup.com/femsec/article/25/4/355/495669
Carlo G. Rizzello, Maria De Angelis, Raffaella Di Cagno, Alessandra Camarca, Marco Silano, Ilario Losito, Massimo De Vincenzi, Maria D. De Bari, Francesco Palmisano, Francesco Maurano, Carmen Gianfrani, Marco Gobbetti. Highly Efficient Gluten Degradation by Lactobacilli and Fungal Proteases during Food Processing: New Perspectives for Celiac Disease. https://journals.asm.org/doi/full/10.1128/AEM.00260-07
Anna Jedrusek-Golinska, Dorota Piasecka-Kwiatkowska, Paulina Zielinska Magdalena Zielinska-Dawidziak, Krystyna Szymandera-Buszka, Marzanna H. Soy Preparations Are Potentially Dangerous Factorsin the Course of a Food Allergy
Yanjun Cong, Fei Lou†, Wentong Xue ,Linfeng Li, Minghai Chena. Characterisation of the IgE-binding immunodominant epitopes on Ara h1. https://www.tandfonline.com/doi/full/10.1080/09540100802172599
Hiroaki Matsuo, Tomoharu Yokooji, Takanori Taogoshi. Common food allergens and their IgE-binding epitopes. Department of Pharmaceutical Services, Hiroshima University Hospital, Hiroshima, Japan. https://www.researchgate.net/publication/282358182
Borinskaya S, N.K. Yankovsky. Structure of prokaryotic_genomes Vavilov Institute of General Genetics November 1999 Molecular Biology 33(6):831-845 https://www.researchgate.net/publication/287462505